rat glial cell line-derived neurotrophic factor (gdnf) elisa kit picokine  (Boster Bio)

Journal: Current Issues in Molecular Biology

Article Title: Expression of G2019S LRRK2 in Rat Primary Astrocytes Mediates Neurotoxicity and Alters the Dopamine Synthesis Pathway in N27 Cells via Astrocytic Proinflammatory Cytokines and Neurotrophic Factors

doi: 10.3390/cimb46050263

Figure Lengend Snippet: Primer sequences.

Article Snippet: The following commercially available ELISA kits were employed: Rat brain-derived neurotrophic factor (BDNF) ELISA Kit PicoKine (EK0308, Boster Biological Technology, Pleasanton, CA, USA), Rat glial cell line-derived neurotrophic factor (GDNF) ELISA Kit PicoKine (EK0363, Boster Biological Technology), Rat Nerve Growth Factor (NGF) ELISA Kit (MBS261790.

Techniques: Sequencing

Journal: Current Issues in Molecular Biology

Article Title: Expression of G2019S LRRK2 in Rat Primary Astrocytes Mediates Neurotoxicity and Alters the Dopamine Synthesis Pathway in N27 Cells via Astrocytic Proinflammatory Cytokines and Neurotrophic Factors

doi: 10.3390/cimb46050263

Figure Lengend Snippet: Levels of neurotrophic factors following ectopic expression of LRRK2 in rASTROs. To validate the changes in protective functions of rASTROs, the levels of neurotrophic factors in the culture media were determined via an mRNA quantification assay ( A ) and an enzyme-linked immunosorbent assay (ELISA) ( B ). These factors included glial cell line-derived neurotrophic factor (GDNF), brain-derived neurotrophic factor (BDNF), and nerve growth factor (NGF). N = 3 (average of duplicate assays); two-way ANOVA with Bonferroni’s post hoc analysis; ns, not significant; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: The following commercially available ELISA kits were employed: Rat brain-derived neurotrophic factor (BDNF) ELISA Kit PicoKine (EK0308, Boster Biological Technology, Pleasanton, CA, USA), Rat glial cell line-derived neurotrophic factor (GDNF) ELISA Kit PicoKine (EK0363, Boster Biological Technology), Rat Nerve Growth Factor (NGF) ELISA Kit (MBS261790.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Derivative Assay

Journal: Current Issues in Molecular Biology

Article Title: Expression of G2019S LRRK2 in Rat Primary Astrocytes Mediates Neurotoxicity and Alters the Dopamine Synthesis Pathway in N27 Cells via Astrocytic Proinflammatory Cytokines and Neurotrophic Factors

doi: 10.3390/cimb46050263

Figure Lengend Snippet: Levels of proinflammatory cytokines following ectopic expression of LRRK2 in rASTROs. To assess neuroinflammation, we examined the levels of proinflammatory cytokines, including inducible nitrogen oxide synthase (iNOS), interleukin-1 β (IL-1β), and tumor necrosis factor α (TNFα), in the culture media via an mRNA quantification assay ( A ) and ELISA ( B ). N = 3 (average of duplicate assays); one-way ANOVA with Bonferroni’s post hoc analysis; ns, not significant; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: The following commercially available ELISA kits were employed: Rat brain-derived neurotrophic factor (BDNF) ELISA Kit PicoKine (EK0308, Boster Biological Technology, Pleasanton, CA, USA), Rat glial cell line-derived neurotrophic factor (GDNF) ELISA Kit PicoKine (EK0363, Boster Biological Technology), Rat Nerve Growth Factor (NGF) ELISA Kit (MBS261790.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay

Journal: Current Issues in Molecular Biology

Article Title: Expression of G2019S LRRK2 in Rat Primary Astrocytes Mediates Neurotoxicity and Alters the Dopamine Synthesis Pathway in N27 Cells via Astrocytic Proinflammatory Cytokines and Neurotrophic Factors

doi: 10.3390/cimb46050263

Figure Lengend Snippet: Changes in the dopaminergic synthesis pathway due to treatment with the CM of transfected rASTROs. ( A , B ) Levels of proteins involved in the dopamine synthesis pathway, including tyrosine hydroxylase (TH), dopamine transporter (DAT), and Nurr1. ( C ) mRNA expression of TH, DAT, and Nurr1 in N27 cells treated with the CM of transfected rASTROs. N = 3; two-way ANOVA with Bonferroni’s post hoc analysis; ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001; #, p < 0.05 based on Student’s t -test; ##, p < 0.01 based on Student’s t -test; ###, p < 0.001 based on Student’s t -test. ( D ) Dopamine levels in the culture media of N27 cells treated with the CM of transfected rASTROs determined using ELISA. N = 4 (average of triplicate assays); one-way ANOVA with Bonferroni’s post hoc analysis; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: The following commercially available ELISA kits were employed: Rat brain-derived neurotrophic factor (BDNF) ELISA Kit PicoKine (EK0308, Boster Biological Technology, Pleasanton, CA, USA), Rat glial cell line-derived neurotrophic factor (GDNF) ELISA Kit PicoKine (EK0363, Boster Biological Technology), Rat Nerve Growth Factor (NGF) ELISA Kit (MBS261790.

Techniques: Transfection, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Cells

Article Title: Peripheral Nerve Regeneration–Adipose-Tissue-Derived Stem Cells Differentiated by a Three-Step Protocol Promote Neurite Elongation via NGF Secretion

doi: 10.3390/cells11182887

Figure Lengend Snippet: Primer sequences.

Article Snippet: The rat NGF Duo Set ELISA Kit (R&D Systems, Minneapolis, MN, USA) and the GDNF (Rat) ELISA Kit (Abnova, Taipei, Taiwan) were used, according to the manufacturers’ instructions.

Techniques:

Journal: Cells

Article Title: Peripheral Nerve Regeneration–Adipose-Tissue-Derived Stem Cells Differentiated by a Three-Step Protocol Promote Neurite Elongation via NGF Secretion

doi: 10.3390/cells11182887

Figure Lengend Snippet: Relative gene expressions after neural differentiation of ASCs with three different protocols. ASCs differentiated with Protocol 1 express the general glial markers S100 and GFAP. ASCs differentiated with Protocol 2 express the markers of the myelinating Schwann cells P0, MBP, and p75. ASCs differentiated with Protocol 3 express the neurotrophins and neurotrophic factors NGF, GDNF, and BDNF.

Article Snippet: The rat NGF Duo Set ELISA Kit (R&D Systems, Minneapolis, MN, USA) and the GDNF (Rat) ELISA Kit (Abnova, Taipei, Taiwan) were used, according to the manufacturers’ instructions.

Techniques:

Journal: Cells

Article Title: Peripheral Nerve Regeneration–Adipose-Tissue-Derived Stem Cells Differentiated by a Three-Step Protocol Promote Neurite Elongation via NGF Secretion

doi: 10.3390/cells11182887

Figure Lengend Snippet: ELISA with supernatants from neuronally differentiated ADSCs. Expression of NGF is especially upregulated after differentiation with Protocol 3. The obtained values were not normalized to the total cell number.

Article Snippet: The rat NGF Duo Set ELISA Kit (R&D Systems, Minneapolis, MN, USA) and the GDNF (Rat) ELISA Kit (Abnova, Taipei, Taiwan) were used, according to the manufacturers’ instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing

Journal: Cells

Article Title: Peripheral Nerve Regeneration–Adipose-Tissue-Derived Stem Cells Differentiated by a Three-Step Protocol Promote Neurite Elongation via NGF Secretion

doi: 10.3390/cells11182887

Figure Lengend Snippet: ELISA: expression of NGF after differentiation with Protocols 1, 2, and 3. The obtained values were not normalized to the total cell number.

Article Snippet: The rat NGF Duo Set ELISA Kit (R&D Systems, Minneapolis, MN, USA) and the GDNF (Rat) ELISA Kit (Abnova, Taipei, Taiwan) were used, according to the manufacturers’ instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing

Journal: Biomaterials Research

Article Title: Peripheral nerve defects repaired with autogenous vein grafts filled with platelet-rich plasma and active nerve microtissues and evaluated by novel multimodal ultrasound techniques

doi: 10.1186/s40824-022-00264-8

Figure Lengend Snippet: Evaluation of neurotrophic factors secreted by nerve microtissues. ELISA detected a rapid increase in the secretion of NGF-β, VEGF, BDNF and GDNF in living nerve microtissues after 3 days of culture. SCs Schwann cells, ELISA Enzyme linked immunosorbent assay

Article Snippet: The concentrations of nerve growth factor-β (NGF-β), vascular endothelial growth factor (VEGF), brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF) were determined using an NGF-β ELISA kit (Boster, EK0471), a VEGF ELISA kit (Boster, EK0540), a BDNF ELISA kit (Boster, 0308) and a GDNF ELISA kit (Boster, EK0363) as instructed by the manufacturer.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Bioactive Materials

Article Title: Acellular nerve xenografts based on supercritical extraction technology for repairing long-distance sciatic nerve defects in rats

doi: 10.1016/j.bioactmat.2022.03.014

Figure Lengend Snippet: Evaluation of the cytotoxicity of the ANXs scaffold in vitro. (A, D) Living/dead double staining of Schwann cells grown on the ANXs scaffold for 3 days and 7 days (live: green, dead: red). (B, E) SEM images of Schwann cells growing on CD SD and CD + scCO 2 NG scaffolds for 7 days (the picture on the right is an enlarged view of the yellow area in the picture on the left). (C, F) Immunofluorescence images of Schwann cells growing on CD SD and CD + scCO 2 NG scaffolds for 7 days, respectively (S100: red, nucleus: blue). (G) Quantification of the number of live/dead double-stained Schwann cells in each region (0.36 mm 2 ). Data are presented as the mean ± SD (n = 3). (H) The CCK-8 assay was performed after 1, 3, 5 and 7 days of cell culture. Data are presented as the mean ± SD (n = 5). (I, J) Quantitative analysis of the GDNF and NGF expression levels of Schwann cells on the ANXs scaffold. Data are presented as the mean ± SD (n = 5). Statistical analysis: n.s. no significances, **p < 0.01, *p < 0.05.

Article Snippet: In brief, the medium of each group was centrifuged at 1500 rpm and 4 °C for 10 min, the concentration of NGF and BDNF in the supernatant was assessed using ELISA kits, the rat GDNF ELISA kit (EK0363, BOSTER, China) and the rat NGF/NGFβ ELISA kit (EK0471, BOSTER, China), and the absorbance of each well at 450 nm was determined using a spectrophotometer (EPOCH TAKE 3, Bio-Tek, USA).

Techniques: In Vitro, Double Staining, Immunofluorescence, Staining, CCK-8 Assay, Cell Culture, Expressing